Science

Introduction When casein paint (a protein in milk) is hydrolysed, the milk turns from cloudy to clear. Trypsin is single of the enzymes up to(p) to do this. Aim To investigate the effect of temperature on the employment of trypsin - exploitation casein as the substrate. Materials and method A piss bath was heat to 25°C. 5cm³ of 4% casein solution was put into one test electron tube and 5cm³ of 0.5% trypsin was added to other test tube. Both were stood in a water bath until they reached the correct temperature. The same measuring of casein solution was added to another(prenominal) test tube and 5cm³ distilled water in a ordinal test tube. This acted as a control. Another control consisted of 5cm³ distilled water in a twenty percent test tube and 5cm³ casein in a lowest test tube. alone of these were also position into the water bath. sear card was placed behind the test tubes to help tarnish the clearing of the solution. The enzyme and substr ate were mixed, a stopwatch started immediately, and the time for the severance to clear tell. This was repeat for the controls, and the whole experiment repeated for antithetic temperatures, ranging from 25°C - 65°C. Results and discussion The control showed no transfigure in falsify at any temperature.

In the interpret below, we plan the relative reaction rate (1/time taken to overcome a colour transfigure) against the temperature. As the temperature increased, the time for a colour change to be seen was less (1/t was bigger). At a certain point, the relative reaction rate started decreasing. This was the optimal temperature (~ 37°C). ! Experiment to determine the rate of reaction of trypsin on casein Click on the next step button to hold back the colour change. Graph depicting the effect of temperature on trypsin activity accomplishable errors The timing may wealthy person been inaccurate.The colour change is subjective. This essence that each person will decide differently when they see a colour change and so everyone would have noted a different time.If you want to get a beneficial essay, order it on our website: OrderCustomPaper.com

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